Selenium Nanoparticles Biosynthesized using Polylophium involucratum (Pall.) Boiss. Seeds Extract and its Antibacterial Activity

Background and Aim : Recently, selenium nanoparticles have emerge as encouraging bactericidal agents, and have been widely applied in various fields of industry such as cosmetic, medical and food industry. In the present project, the biosynthesis of selenium nanoparticles was conducted using the seeds of extract of Polylophium involucratum (Pall). Boiss., and investigation of its antibacterial properties.Methods : In this project the biosynthesis of selenium nanoparticles using hydroalcohlic extract of seeds of Polylophium involucratum (Pall). Boiss. as a reducing agent by microwave irradiation method. The synthesized selenium nanoparticles were characterized using various instrumental techniques. Later, the antibacterial activity of the synthesized selenium nanoparticles was tested by (Disk Diffusion Method) using both gram positive as well as gram negative bacteria i.e. Staphylococcus aureus (PTCC ۱۱۱۳), Bacillus subtilis (PTCC ۱۱۵۶) and Escherichia coli (PTCC ۱۳۹۹) respectively. Results : The phytochemical compounds play a vital role in the nanoparticles synthesis was identified using the UV-Vis, FT-IR, XRD, and TEM. The synthesized selenium nanoparticles hydroalcohlic extract of seeds of Polylophium involucratum (Pall). Boiss. exhibited good antibacterial potential against gram positive and gram negative bacterial strains. Also, our preliminary phytochemical analysis of seeds extract using hydroalcohlic as solvent confirmed the presence of (Flavonoids, Terpenoids, Coumarins, Phenols, Cardiac glycosides, Quinones and Saponins). This indicates that antibacterial activities may be due to presence of secondary metabolites.Conclusion : The synthesized selenium nanoparticles exhibited good antibacterial potential against gram positive and gram negative bacterial strains. Further, efficient antibacterial activity of the synthesized selenium nanoparticles proves the application potential of green synthesis in the area of nano-medicine.

Keywords:

Selenium nanoparticles , Polylophium involucratum (Pall). Boiss. , Staphylococcus aureus , Nano-Medicine.