Umbelliprenin induced both anti-inflammatory and regulatory cytokines in C۵۷/BL۶ mice

Objective(s): Umbelliprenin is a prenyloxy-coumarin with pharmacologically polyvalent activity. Several studies have shown its anti-inflammatory, anti-tumor, antioxidant, and antigenotoxic activity, and other functions. However, the exact mechanism of action of this compound on the immune response has not yet been shown. Here, we investigated umbelliprenin effects on the predominance of Th۱ and Th۲ responses in normal C۵۷/BL۶ mice. Materials and Methods: Umbelliprenin (۲.۵ mg/۲۰۰ µl IP) were administered to six C۵۷/BL۶ mice every other day for ۸ days. Paraffin and PBS-injected mice were enrolled as solvent and control groups, respectively (n=۶ mice/group). IL-۱۰, IFN-γ, and IL-۴ levels were determined in sera and also in splenocytes culture supernatants in the presence of Con A (۳ µg/ml) after ۷۲ hr. H&E staining of paraffin embedded blocks was performed for lung and liver tissues of mice. Results: Umbelliprenin could significantly increase the secretion of IFN-γ and IL-۴ in sera and IL-۱۰ in splenocytes cultures. Comparison of IFN-γ /IL-۴ in the sera and splenocytes culture supernatants showed lower ratios in umbelliprenin treated mice than in solvent and untreated groups. Conclusion: The in vivo study showed that umbelliprenin could induce anti-inflammatory responses via the predominance of Th۲ cells and some regulatory responses in C۵۷/BL۶ mice.

Keywords:

Anti-inflammatory , Concanavalin A (Con A) , Interferon gamma (IFN-γ) , Prenyloxy-coumarin , Umbelliprenin