CFP۱۰: mFcγ۲ as a novel tuberculosis vaccine candidate increases immune response in mouse

Publish Year: 1396
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJBMS-20-2_003

تاریخ نمایه سازی: 28 مهر 1400

Abstract:

Objective(s): Despite treatment with antibiotics and vaccination with BCG, tuberculosis (TB) is still considered as one of the most important public health problems in the world. Therefore, designing and producing a more effective vaccine against TB seems urgently. In this study, immunogenicity of a fusion protein which consisting or comprising CFP-۱۰ from Mycobacterium tuberculosis and the Fc-domain of  mouse IgG۲a was evaluated as a novel subunit vaccine candidate against TB. Materials and Methods: The genetic constructs were cloned in pPICZαA expression vector and recombinant vectors (pPICZαA-CFP-۱۰: Fcγ۲a and pPICZαA-CFP-۱۰:His) were transformed into Pichia pastoris. To evaluate the expression of recombinant proteins, SDS-PAGE and immunoblotting were used. The immunogenicity of recombinant proteins, with and without BCG were assessed in BALB/c mice and specific cytokines against recombinant proteins (IFN-γ, IL-۱۲, IL-۴, IL-۱۷ and TGF-β) were evaluated. Results: The levels of IFN-γ and IL-۱۲ in mice that received recombinant proteins was higher than the control groups (BCG and PBS). Thus, both recombinant proteins (CFP-۱۰:Fcγ۲a and CFP-۱۰:His) could excite good response in Th۱-cells. The Fc-tagged protein had a stronger Th۱ response with low levels of IL-۴, as compared to CFP-۱۰:His. However, the highest level of Th۱ response was observed in groups that were vaccinated with BCG (prime) and then received recombinant protein CFP-۱۰: Fcγ۲a (booster). Conclusion: The results demonstrated that binding mice Fc-domain to CFP-۱۰ protein can increase the immunogenicity of the subunit vaccine. Further studies, might be able to design and produce a new generation of subunit vaccines based on the Fc-fused immunogen.

Authors

Ali Asghar Baghani

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Saman Soleimanpour

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Hadi Farsiani

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Arman Mosavat

HIV/AIDS, HTLV and Viral Hepatitis Research Center, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad, Iran

Masoud Yousefi

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Zahra Meshkat

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Seyed Abdolrahim Rezaee

Immunology Research Center, Inflammation and Inflammatory Diseases Division, Medical school, Mashhad University of Medical Sciences, Mashhad, Iran

Saeid Amel Jamehdar

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Mohammad Reza Akbari Eydgahi

Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran

Hamid Sadeghian

Organic Chemistry, Department of Laboratory Sciences, Mashhad University of Medical Sciences, Mashhad, Iran

Kiarash Ghazvini

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

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  • ۱.World Health Organization. Global tuberculosis report ۲۰۱۵ (WHO). ۲۰۱۵.Available at: ...
  • Nuttall JJ, Davies MA, Hussey GD, Eley BS. Bacillus calmette-guerin ...
  • Logan KE, Chambers MA, Hewinson RG, Hogarth PJ. Frequency of ...
  • O'Garra A, Redford PS, McNab FW, Bloom CI, Wilkinson RJ, ...
  • Liljeqvist S, Ståhl S. Production of recombinant subunit vaccines: protein ...
  • Guo S, Xue R, Li Y, Wang SM, Ren L, ...
  • Guinn KM, Hickey MJ, Mathur SK, Zakel KL, Grotzke JE, ...
  • Ganguly N۱, Siddiqui I, Sharma P. Role of M. tuberculosis ...
  • Ottenhoff TH, Kaufmann SH. Vaccines against Tuberculosis: where are we ...
  • Soleimanpour S, Hassannia T, Motiee M, Amini AA, Rezaee SA. ...
  • Soleimanpour S, Farsiani F, Mosavat A, Ghazvini K, Eydgahi MR, ...
  • Adamova E, Walsh MC, Gosselin DR, Hale K, Preissler MT, ...
  • Keler T, Guyre PM, Vitale LA, Sundarapandiyan K, van De ...
  • den Haan JM, Lehar SM, Bevan MJ. CD۸ (+) but ...
  • Laemmli UK. Cleavage of structural proteins during the assembly of ...
  • Mosavat A, Soleimanpour S, Farsiani H, Sadeghian H, Ghazvini K, ...
  • Davidsen J, Rosenkrands I, Christensen D, Vangala A, Kirby D, ...
  • Farsiani H, Mosavat A, Soleimanpour S, Sadeghian H, Akbari Eydgahi ...
  • Flynn JL, Chan J, Triebold KJ, Dalton DK, Stewart TA, ...
  • Herbst S, Schaible UE, Schneider BE. Interferon gamma activated macrophages ...
  • Méndez-Samperio P. Role of interleukin-۱۲ family cytokines in the cellular ...
  • Torrado E, Cooper AM. IL-۱۷ and Th۱۷ cells in tuberculosis. ...
  • Harris J, De Haro SA, Master SS, Keane J, Roberts ...
  • Ansel KM, Lee DU, Rao A. An epigenetic view of ...
  • Wilson CB, Rowell E, Sekimata M. Epigenetic control of T-helpercell ...
  • Wu Y, Woodworth JS, Shin DS, Morris S, Behar SM. ...
  • Li W, Deng G, Li M, Zeng J, Zhao L, ...
  • Braren I, Greunke K, Umland O, Deckers S, Bredehorst R, ...
  • Guo Y, Kang W, Zhong Y, Li R, Li G, ...
  • Li P, Anumanthan A, Gao XG, Ilangovan K, Suzara VV, ...
  • Reljic R, Paul MJ, Arias MA. Cytokine therapy of tuberculosis ...
  • Lockhart E, Green AM, Flynn JL. IL-۱۷ production is dominated ...
  • Dalmia N, Ramsay AJ. Prime–boost approaches to tuberculosis vaccine development. ...
  • Husain AA, Warke SR, Kalorey DR, Daginawala HF, Taori GM, ...
  • Konduru K, Bradfute SB, Jacques J, Manangeeswaran M, Nakamura S, ...
  • Lu L, Palaniyandi S, Zeng R, Bai Y, Liu X, ...
  • Mekhaiel DN, Czajkowsky DM, Andersen JT, Shi J, El-Faham M, ...
  • Loureiro S, Ren J, Phapugrangkul P, Colaco CA, Bailey CR, ...
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