Gestational diabetes leads to down-regulation of CDK۴-pRB-E۲F۱ pathway genes in pancreatic islets of rat offspring
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 20، Issue: 2
Publish Year: 1396
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-20-2_007
تاریخ نمایه سازی: 28 مهر 1400
Abstract:
Objective(s): The link between a hyperglycemic intrauterine environment and the development of diabetes later in life has been observed in offspring exposed to gestational diabetes mellitus (GDM), but the underlying mechanisms for this phenomenon are still not clear. Reduced β-cells mass is a determinant in the development of diabetes (type ۱ and type ۲ diabetes). Some recent studies have provided evidence that the CDK۴-pRB-E۲F۱ regulatory pathway is involved in β-cells proliferation. Therefore, we postulated that GDM exposure impacts the offspring’s β-cells by disruption in the CDK۴-pRB-E۲F۱ pathway. Materials and Methods: Adult Wistar rats were randomly allocated in control and diabetic group. The experimental group received ۴۰ mg/kg/body weight of streptozotocin (STZ) on day zero of gestation. After delivery, diabetic offspring of GDM mothers and control dams at the age of ۱۵ week were randomly scarified and pancreases were harvested. Langerhans islets of diabetic and control groups were digested by collagenase digestion technique. After RNA extraction, we investigated the expressions of the kir ۶.۲ and CDK۴-pRB-E۲F۱ pathway genes by quantitative real-time PCR. Results: GDM reduced the expression of CDK۴-pRB-E۲F۱ pathway genes in Langerhans islets cells of offspring. CDK۴, pRB and E۲F۱ pathway genes were downregulated in diabetic islets by ۵۱%, ۳۵% and ۸۴%, respectively. Also, the expression of Kir ۶.۲ was significantly decreased in diabetic islets by ۸۸%. Conclusion: We suggest that the effect of gestational diabetes on offspring’s β-cells may be primarily caused by the suppression of CDK۴-pRB-E۲F۱ pathway.
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Authors
Zahra Nazari
Department of Animal Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Mohammad Nabiuni
Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Mohsen Saeidi
Stem Cell Research Center, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
Mohammad Jafar Golalipour
Gorgan Congenital Malformations Research Center, Depatment of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran
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