Down-regulation of HSP۴۰ gene family following OCT۴B۱ suppression in human tumor cell lines

Objective(s): The OCT۴B۱, as one of OCT۴ variants, is expressed in cancer cell lines and tissues more than other variants and plays an important role in apoptosis and stress (heat shock protein) pathways. The present study was designed to determine the effects of OCT۴B۱ silencing on expressional profile of HSP۴۰ gene family expression in three different human tumor cell lines. Materials and Methods: The OCT۴B۱ expression was suppressed by specific siRNA transfection in AGS (gastric adenocarcinoma), ۵۶۳۷ (bladder tumor) and U-۸۷MG (brain tumor) cell lines employing Lipofectamine reagent. Real-time PCR array technique was employed for RNA qualification. The fold changes were calculated using RT۲ Profiler PCR array data analysis software version ۳.۵. Results: Our results indicated that fifteen genes (from ۳۶ studied genes) were down-regulated and two genes (DNAJC۱۱ and DNAJC۵B) were up-regulated in all three studied tumor cell lines by approximately more than two folds. The result of other studied genes (۱۹ genes) showed different expressional pattern (up or down-expression) based on tumor cell lines. Conclusion: According to the findings of the present study, we may suggest that there is a direct correlation between OCT۴B۱ expression in tumor cell lines (and tissues) and HSP۴۰ family gene expressions to escape from apoptosis and cancer expansion.

Keywords:

HSP۴۰ gene family , OCT۴B۱ , siRNA , Tumor cell lines