Effect of normobaric hyperoxia on gentamicin-induced nephrotoxicity in rats
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 17، Issue: 4
Publish Year: 1393
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-17-4_009
تاریخ نمایه سازی: 5 آبان 1400
Abstract:
Objective(s):Gentamicin sulphate (GS) nephrotoxicity seems to be related to the generation of reactive oxygen species. There is evidence that oxygen preconditioning increases the activity of antioxidant enzymes. Materials and Methods: Forty eight female rats were divided into ۶ groups (n=۸) as follows: group ۱ was the control, group ۲ received daily GS, groups ۳,۴ and ۵ received oxygen ۲ hr/day for ۲ days, ۴ hr/day for ۲ days, ۴ hr/day for ۴ days, recpectively and then received daily GS, group ۶ received oxygen ۲ hr/day for ۲ days and then received ۲ hr oxygen before daily GS injection. Oxygen (with ۹۰% purity) used at the flow rate of ۴ l/min. GS administred for ۸ days (۱۰۰ mg/kg, IP). Tissue sections prepared from the left kidney, stained with PAS method and then studied hisopathologically and stereologically. The right kidneys were homogenized and the supernatants were prepared. Serum MDA, creatinine and urea, renal MDA, gluthatione and catalase activity were measured. The data were analyzed by Mann-Whitney U test at the significant level of PResults: Oxygen therapy significantly improves serum creatinine and urea, preserve tubular volume density, reduce tubular necrosis in groups ۴ and ۶ compared to group ۲. Oxygen therapy significantly increases renal catalase in groups ۴ and ۶ compared to group ۲. Conclusion: Pretreatment with normobaric hyperoxia and daily oxygen therapy improved gentamicin nephrotoxicity possibly via inhibition of lipid peroxidation and increasing the renal catalase activity but could not restore any parameter at the same levels as control group.
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Authors
Majid Tavafi
Department of Anatomy, Lorestan University of Medical Sciences, Faculty of Medicine, Khoram Abad, Iran
Hasan Ahmadvand
Razi Herbal Drugs Research Center, Lorestan University of Medical Sciences, Khoram Abad, Iran
Ahmad Tamjidipour
Department of Anatomy, Lorestan University of Medical Sciences, Faculty of Medicine, Khoram Abad, Iran
Bahram Rasolian
Department of Physiology, Lorestan University of Medical Sciences, Faculty of Medicine, Khoram Abad, Iran
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