ABCG۵ gene responses to treadmill running with or without administration of Pistachio atlantica in female rats

  Objective(s): ABC transporters comprise a large family of transmembrane proteins that use the energy provided by ATP hydrolysis to translocate a variety of substrates across biological membranes. All members of the human ABCG subfamily, except for ABCG۲, are cholesterol-transporter. The aim of this study was to determine the liver, the small intestine and kidney ABCG۵ relative gene expression in response to treadmill-running training in female rats. Materials and Methods: Twenty Wistar rats (۶-۸ weeks old and ۱۲۵-۱۳۵ g weight) were used. Animals were randomly assigned to saline-control (SC), saline-training (ST), and Baneh-control (BC), and Baneh-training (BT) groups. Training groups did the exercise on a motor-driven treadmill at ۲۵ m/min (۰% grade) for ۶۰ min/day for eight weeks (۵ days/week). Rats were fed orally, with Baneh extraction and saline for six weeks. The two-way ANOVA was employed for statistical analysis.  ABCG۵ relative gene expression was detected by Real-time PCR method. Results:The current findings indicate that the Baneh-treated tissues had significantly lower levels of ABCG۵ gene expression in the liver, small intestine, and kidneys (P< ۰.۰۰۱, P< ۰.۰۰۳, P< ۰.۰۰۱, respectively), when compared with saline-treated tissues. However, a higher level of gene expression was observed in exercise groups. A lower level of HDL-c but not triglyceride (TG) and total cholesterol (TC) levels were found in Baneh-treated animals at rest. Conclusion: Exercise training increases ABCG۵ relative gene expression in the liver, small intestine and kidney tissues; therefore exercise training may adjust the reduction of ABCG۵ relative gene expression in Baneh-training group.

Keywords:

ABCG۵ , ABC transporters , Female rats , Pistachia atlantica , Treadmill exercise