Bioaccumulation and the Effect of Selenate Concentration on Growth and Photosynthetic Pigment Content of Spirulina platensis

Selenium (Se) is a necessary microelement for microalgae growth. Moreover, it might directly act and cause a decrease in primary production in microalgae. Nowadays, there is a great focus on preparations of Se-enriched products. In the current study, the effects of various selenate concentrations on Spirulina were evaluated as a primary stage to large-scale production of Se-enriched S. platensis. Zarrouk medium supplemented to investigate the stimulatory/inhibitory effects of selenate on growth at various concentrations. Biomass dry weight and cell mass were measured on OD۵۵۰. Thereafter, inhibitory and algicidal concentrations were determined. Furthermore, its effects on morphology and changes of some important pigments in response to the metal challenge were investigated, too. While the results showed that at ۵ and ۱۰ ppm concentrations growth was supported, the dry weight of microalgae decreased at selenate levels above ۵۰ ppm. Besides, the inhibitory and lethal effects of selenate were at ۱۰۰ ppm and ۳۰۰ ppm, respectively. In addition, morphological changes were observed at this concentration. Additionally, chlorophyll, carotenoid, and phycobiliproteins showed a stimulatory effect at ۵-۵۰ ppm, ۱۰ ppm, and ۱۰ ppm, respectively. Moreover, Spirulina uses for foods production because some chemicals are unique compounds. Simple cultivation method and high quality of its protein, and no toxic effects, cause its feasibility for large-scale production.

Keywords:

Spirulina platensis , Selenate , Photosynthetic Pigment Content , Toxicity