The effect of allicin in garlic extract on the viability of gastric cancer cell line (AGS)

Background and Aim: Various studies show that plant extracts can have cytotoxic effects on cancer cells. The aim of these studies was to investigate the effect of allicin in garlic extract on the survival of gastric cancer cells in culture medium. material and methods: In this experiment, two cell lines were used. One of them is a cancer cell line (AGS) that was cultured in DMEM / F ۱۲medium containing %۱۰ Fetal bovine serum and the other one is a healthy cell line (HuGu) that was cultured in DMEM / F ۱۲medium containing %۱۵fetal bovine serum and ۲Percent of MEM medium and stored in an incubator at ۳۷° C and %۵CO ۲pressure. then the cell lines were randomly divided to control and exposed groups of ۵, ۴, ۳, ۲, ۱, ۰.۶, ۰.۴, ۰.۲, ۰.۱, ۰.۰۸.۰.۰۴, ۰.۰۲, and ۰.۰۱mg/ml of allicin in garlic extract to determine cell viability by using MTT assay. In the end, data were analyzed by one-way analysis of variance.Results: The survival of the AGS cell line was significantly different between the control group and different doses of allicin and the survival of a healthy cell line (HuGu) was higher than that of the cancer cell line.Conclusion: The results showed that allicin affects the survival of the cancer cell line and causes the destruction of cancer cells.

Keywords:

Allicin , garlic extract , viability , gastric cancer , AGS