The effect of allicin in garlic extract on BAX and Bcl۲ gene expression in gastric cancer cell line (AGS)

Background and Aim: Gastric cancer is the fourth most common cancer in men and the fifth most common cancer in women, and approximately two-thirds of it is in developing countries. it happens. In cancer, the balance between cell proliferation and apoptosis is disturbed, resulting in a direct link between the expression of BAX and BCL۲ genes and There is a process of becoming cancerous. This study aims to determine the extent to which the expression of these genes changes in the allicin-treated gastric cancer cell line. Methods: In this experiment, two cancer cell lines (AGS) were used in DMEM / F۱۲ medium containing ۱۰% fetal bovine serum. And normal cell line (HuGu) cultured in DMEM / F۱۲ medium containing ۱۵% fetal bovine serum and ۲% MEM culture medium Were stored in an incubator at ۳۷ ° C and ۵ %CO۲. Then the expression of the GAPDH gene as control and BAX and Bcl۲ were analyzed and the data were obtained using SPSS statistical software and ANOVA and T_Test statistical tests. Was analyzedResults: The dose of allicin used in this experiment had no significant effect on the expression of BAX and Bcl۲ genes in two normal and cancerous samples. While decreasing BAX gene expression and increasing Bcl۲ expression were expected Conclusion: According to previous research and the present study, allicin may be more effective at higher doses

Keywords:

AGS , HuGu , gastric cancer , allicin , garlic extract , BAX , BCL-۲ , gene expression , RT-PCR