Evaluation of Antibacterial and Antioxidant effect of Ziziphora tenuior essential oil, native to Zanjan province.

In the present investigation, the chemical compositions and evaluation of Antibacterial and Antioxidant effect of Ziziphora tenuior essential oil were examined. The chemical compositions analyzed with GC–MS. The antibacterial activity of essential oil of Ziziphora tenuior was tested against Bacillus subtilis, Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus epidermidis, Klebsiella pneumonia by agar well diffusion method, Minimum inhibitory concentrations (MICs) and Minimum bactericidal concentration (MBC). Ziziphora tenuior had several level of antibacterial activities (MIC>۰.۶۲۵ μg/mL & MBC>۱.۲۵ μg/mL). Antioxidant activity of essential oil of Ziziphora tenuior were determined by total phenolic with ۱۶۹.۳۳ ± ۷.۰۲ GAE mg/g, DPPH- radical-scavenging activities with significant differences (P<۰.۰۵) among IC۵۰ ۷۹۸.۷۱ ± ۷.۹۱ mg/L comparable to garlic acid (۶۱.۲۰ ± ۱.۶۷ mg/L), Ferric reducing antioxidant power assay (FRAP) with significant differences (P<۰.۰۵) among Absorption of essential oil comparable to standard (ascorbic acid), Hydroxyl Radical Scavenging Activity (HRSA) with significant differences (P<۰.۰۵) among IC۵۰ of essential oil of Ziziphora tenuior (۸.۰۱ ± ۰.۱۷ mg/mL) comparable to ascorbic acid (۰.۱۲ ± ۰.۰۰۵ mg/mL) and Ferrous ion chelation with significant differences (P<۰.۰۵) among IC۵۰ of essential oil of Ziziphora tenuior (۷۹.۵۴ ± ۲.۹۸ μg/mL) comparable to EDTA (۰.۷۲ ± ۰.۰۱۵ μg/mL). Ziziphora tenuior in all determining methods had several level of antioxidant and antibacterial activities.

Keywords:

Antibacterial , Antioxidant , MIC , MBC , phenolic content , DPPH , FRAP , HRSA , Ferrous ion chelation , Zanjan , Ziziphora tenuior.