Cytotoxicity and Apoptosis Induction of ۷- Hydroxycoumarin on SW۴۸۰ Colon Cancer Cell Line

Introduction: Colorectal cancer is a common disease ranked as the third-highest deathly cancer worldwide. In recent years, for the treatment of cancers scientists have preferred herbal remedies because of their better effects and fewer side effects. So, in this study, the cytotoxicity effect and apoptosis induction of ۷-hydroxycycoumarin were investigated on the SW۴۸۰ colon cancer cell line.Methods: anti-proliferative effect of ۷-hydroxycoumarin was investigated using Trypan blue and MTT assay at different concentrations on SW۴۸۰ cell line. Additionally, apoptosis induction was examined by flow cytometry and real-time PCR.Results: The results show a decrease in viability. IC۵۰ concentrations were observed at the concentrations of ۱۶۴.۲, ۸۰.۴۸, and ۴۱.۹۷ μg/ml for ۲۴, ۴۸, and ۷۲ h, respectively. Of note, IC۵۰ concentrations were not observed in the trypan blue assay. As well, there was a ۲.۴-fold increase in apoptosis (P<۰.۰۰۱) using Annexin / PI kit by flow cytometry. as well as the increased expression of BAX/BCL۲ ratio of about ۱۳.۶۹, which was observed at ۱۰۰ μg/ml compared to the control.Discussion: The results indicate that ۷-hydroxycoumarin could inhibit cell proliferation and induce apoptosis in the SW۴۸۰ cell line. Therefore, ۷-hydroxycycoumarin can be considered as an anti-cancer agent in the complementary treatment of colon cancer.

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Keywords: Apoptsis , colon cancer , Cytotoxicity , ۷- hydroxycoumarin