Research Article: Comparative effect of different vaccines on immune-related gene expressions of rainbow trout (Oncorhynchus mykiss) and experimentally infected with Streptococcus iniae and Lactococus garvie
Publish place: Iranian Journal of Fisheries Sciences، Vol: 20، Issue: 5
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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JR_JIFRO-20-5_011
تاریخ نمایه سازی: 23 بهمن 1400
Abstract:
To understand how different vaccines, i.e., bivalent formalin-inactivated vaccine (Lactococus garvie and Streptococcus iniae), could affect the immune-related gene expressions, the TNF-alpha, IL-۱β and IL-۸ genes of head kidney was measured in Oncorhynchus mykiss. To address this, ۶۳۰ juvenile fish weighing ۲۶±۳ g were initially (day ۰) injected by three different treatments, including control group (normal saline only), AquaVacTM Garvetil (Merck) and Iranian vaccine (Jehad-e-Daneshagahi) and followed by bath immersion into the same vaccine on day ۳۰. The head kidney was withdrawn to measure immune-related gene expressions at the beginning of experiment (day ۰), and following ۱۴, ۳۰, ۴۵ and ۶۰ days of post-vaccination. In the next step, control and both vaccinated groups were also subjected to either L. garvie or S. iniae challenge. Similar samples were taken immediately following bacteria injection (time= ۰) and ۱۲, ۴۸, ۷۲ h, and following ۷ and ۱۰ days of challenged test. The real-time PCR indicated up-regulation of all cytokine genes following vaccination at day ۱۴ as compared to initial day (p<۰.۰۵). In pre-challenged experiment, the higher induction in the level of those genes in the head kidney was related to AquaVac vaccine (p<۰.۰۵). Injection by S. iniae and L. garviae induced the level of immune-related gene expression in the head kidney within the first few days with higher intensity in case of unvaccinated control group. Although the AquaVac produces higher up-regulation of inflammatory gene expression rather than Iranian vaccine, the immunostimulatory effects of both vaccines is a time-restricted.
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Authors
H. Khaj
Razi Vaccine and Serum Research Institute, Agriculture Research Education and Extension Organization, AREEO, Karaj, Iran
M. Mesbah
Shahid Chamran University of Ahvaz
M.R. Tabandeh
Shahid Chamran University of Ahvaz
T. Mohammadian
Shahid Chamran University of Ahvaz
M. Dadar
Shahid Chamran University of Ahvaz
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