Research Article: Single cell production by Claveromycice frajilice and Fusarium oxysporum in Kilka stick water

The production of single cell protein (SCP) is one of the cheap protein sources for use in aquaculture. In this study, Claveromycice frajilice and  Fusarium oxysporum were used to produce SCP using Kilka stick water as the source of medium. Adaptation of C. frajilice and F. oxysporum to pure stick water was performed by increasing the concentration of stick water in distilled water. Treatments included ۵۰% and ۱۰۰% stick water, and a group without stick water as control. The pattern of the yeast and fungus growth was studied by spectrophotometry at ۶۰۰ nm. The final product was analyzed for values of dry materials, amino acids profiles, total protein, moisture and ash. The results showed protein production by C. frajilice in ۵۰% and ۱۰۰% stick water was ۵۵.۳۵% and ۵۷.۴۷%, respectively, compared to ۵۴.۶۵% in control group (P>۰.۰۵). Protein production using F. oxysporum was ۵۳.۱۷% and ۵۴.۳۹±۰.۴۵% in ۵۰% and ۱۰۰% stick water, respectively compared to ۴۹.۷۱% in control group (P>۰.۰۵).  The results showed that amino acids composition in produced SCP was comparable with the suggested profiles of requirement by FAO/WHO and NRC. Based on to the obtained results, application of pure Kilka stick water is suitable for production of C. frajilice as the source of SCP.

Keywords:

Claveromycice frajilice , Fusarium oxysporum , SCP , Stick water , Kilka fish meal.