Densitometric study of extraction site healing in rats

Aim: A complete knowledge of bone healing is a prerequisite to many decision makings in dentistry and is considered as a basic event in some research strategies. Any method that can accurately monitor the healing process is important to clinician providing the information required for further treatment planning. Our study shows a simple and reliable method to evaluate the events that occur during socket healing in rats.Materials and Methods: Thirty five "Sprague - dawly" male rats were selected. All rats were anesthetized with intra peritoneal injection of Ketamin ۱۰% and Xylazine ۲% at the dose of ۲ mUkg on day I. Mandibular left first molars were extracted with the use of very small excavator and hemostat forceps. At the same day,۷,۱۴,۲۱,۲۸,۴۵ and ۶۰ days after extractions, five rats were sacrificd, the left mandibles were removed and placed into ۱۰% Formalinc. Muscles and gingival tissue were dissected away the Following day and the mandible was placed on a radiographic film and the buccul views were taken. The radiographs were scanned and analyzed with Avcnd (program that designed in matlab ۶.۱).Results: Increased density of the image of apical and crestal areas of the socket was observed on day ۷. The maximum density of apical area was reached on day ۲۸ and preceded towards crestal area on day ۶۰. Density increasing in the crestal area is slower than apical area.Conclusion: Densitometrie evaluation of extraction socket healing using Avend program is useful and reliable to objectively assess bone formation and mineralization.

Keywords:

Densitometry , Animal study , Extraction Site , healing