Evaluation of DNA vaccine encoding HPV-۱۶ E۷ on Lymphocyte proliferation induction in Human Papilloma Virus-associated tumor animal models

Background & Objective: Human papillomavirus (HPV) oncoproteins, including E۶ and E۷ are constitutively expressed in cervical cancer cells. These proteins are ideal targets to be used for developing therapeutic vaccines against existing HPV-associated carcinomas. The aim of this study was to measure the proliferation response rate of splenic lymphocytes derived from E۷-HPV۱۶ encoding plasmid injection on the tumor mouse model of papillomavirus. Method: C۵۷BL/۶ mice were inoculated subcutaneous with ۵× ۱۰⁵ TC-۱ cells in three times with two weeks intervals and then immunized with HPV-۱۶ E۷ DNA vaccine. The proliferation response of splenic cells was measured by MTT assay. IL۱۲ cytokine was measured by ELISA assay and the mass of tumor was calculated with caliper for six weeks. Results: Following the application of DNA vaccines containing E۷ therapeutic gene, the proliferative response of splenic cells was provoked significanltly higher than the stimulation in control group (P<۰.۰۵). Moreover, the secretion of IL۱۲ was significantly increased in vaccinated mice tumor tissue (P<۰.۰۵). The growth of tumor in vaccinated group was markedly decreased in comparison to PBS and pcDNA۳ groups (P<۰.۰۵). Conclusion: Our findings revealed that the application of DNA vaccine containing E۷ gene in a tumor mouse model may induce anti-tumor cellular immune responses.

Keywords:

DNA vaccine , Papillomavirus tumor model , Lymphocyte proliferation assay , HPV , DNA واکسن , مدل موش توموری پاپیلوماویروس , پاسخ تکثیری لنفوسیت های CD۴ , پاپیلوماویروس