The Study of the Effect of Nickel Heavy Metal, Salinity and temperature on Production of Alkaloid in Catharantus Roseus Medicinal Plant

Plants, in their life cycle, are usually exposed to various kinds of environmental stresses including Heavy Metals, salinity and heat shok which affects many physiological parameters of plants. Catharantus Roseus plant, as a rich source of alkaloid chemical compounds consists of more than ۱۳۰ secondary metabolites. Some of these compounds have pharmacological effects. Vinbelastine which is the most important alkaloid found in the leaves of Catharantus Roseus is used to heal the diseases like: testicle and breast cancers, lymphoma, neuroblastoma, hodgkin and nonhodgkin’s lymphoma, Mycosis fungoides, histiocytosis and kaposi’s sarcoma. It also controls mitotic activity by stopping the cells in metaphase through nonreturnable connection to tubeline. This alkaloid is the chemical analog of vincristine. In this research, the effect of ۰, ۲.۵, ۵, ۱۰, ۲۵, ۵۰ mM density of NiCl۲ and ۰, ۲۵, ۵۰, ۱۰۰, ۲۰۰ mM density of NaCl and temperature of ۲۰°C, ۲۵°C, ۳۰°C and ۳۵°C on the amount of alkaloids of Catharantus Roseus’s total seeds of pink and white variety was investigated. The results showed that The total amount of alkaloid noticeably increased in the plant root and shoot. Investigating the TLC plates/ planes, the increase in the amount of vinbelastine, katharantine, and ajmalicine alkaloids especially in high densities and temperatures was observed.

Keywords:

Catharantus Roseus , nickel heavy metal , Salinity , Temprature , Alkaloids