Effects of kisspeptin incubation on the mature mouse testicular sperms quality: An experimental study

Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJRM-20-4_006

تاریخ نمایه سازی: 18 اردیبهشت 1401

Abstract:

Background: Sperm quality has an important role in the success of assisted reproductive techniques, by adding some bioactive agents with a positive impact on sperms, it can be improved. Objective: This study aimed to evaluate the effects of kisspeptin on the sperm motility criteria, Lactate dehydrogenase-C (LDHC) activity, acrosomal reaction, and capacitation in the mouse testicular sperm in vitro. Materials and Methods: Sperm samples were extracted from testes of ۹۶ male Balb/C mice weighing ۲۵-۳۰ gr, aged ۶-۸ wk. Then, they were separated into ۴ parts; ۲ controls and ۲ kisspeptin-treated aliquots; each one incubated for either ۱۵ or ۳۰ min. The sperm motility and the LDHC activity were evaluated, and also the frequency of the non-capacitated, intact, and acrosomal-reacted sperms were evaluated by staining with Wheat germ agglutinin, Peanut agglutinin, and Concanavalin A, respectively. The stained sperms were analyzed by flow cytometry and fluorescent microscope. Results: Our result showed that kisspeptin increased both the sperm motility (p = ۰.۰۴) and LDHC enzyme activity (p = ۰.۰۴) after ۱۵ min of incubation. At the same time, it did not impact the frequency of the non-capacitated, intact and acrosomal-reacted sperms after incubation in the same period (p = ۰.۱۶). Conclusion: A ۱۵ min period of incubation with kisspeptin could be applicable for evaluating sperm motility and LDH activity.

Authors

Masoumeh Akmali

Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Roghayeh Yalmeh

Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Tahereh Talaei-Khozani

Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Fatemeh Karimi

Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Elham Aliabadi

Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

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