The effects of sodium chloride stress on some biochemical characteristics and antioxidative enzymes activities in two sunflower (Helianthus annuus L.) genotypes

Salinity is one of the most important non-biological stresses that affect plant growth and development. Effect of different levels of NaCl (۰, ۲, ۴, ۶ and ۸ dS/m) were investigated on enzymatic and non-enzymatic activities in C۶۴ and C۶۸ oilseed sunflower genotypes at two times; ۳ and ۱۲ days after salinity stress application. Net photosynthesis rate, chlorophyll content and soluble proteins amount decreased by increasing salinity level but proline and malondialdehyde (MDA) contents increased. However, the changes in net photosynthesis in the two studied genotypes was different across time and do not follow statistically the same trend line. In genotype C۸۶, the reduction of photosynthesis rate at all studied salinity levels was very high compared to normal condition (۰ dS/m) after ۳ days; especially at salinity levels of ۲, ۴ and ۶ dS/m, while ۱۲ days later, the decrease of photosynthesis rate was moderate at salinity levels of ۲ and ۴ dS/m but severe at ۶ and ۸ dS/m salinity levels. The highest amount of proline (۳۱.۳۶%) related to tolerant genotype and the lowest amount (۷.۷۲%) related to susceptible one was measured ۱۲ days after ۲ dS/m salt stress treatment. Considerable MDA was observed in both tolerant and sensitive genotypes ۱۲ days post salt stress application; the highest amount (۸۳%) was observed at ۸ dS/m treatment. Catalase and ascorbate peroxidase activity increased with increasing salt intensity. The rate of increase in guaiacol peroxidase activity was higher in C۸۶ genotype than C۶۴. Chlorophyll a and total chlorophyll contents decreased in both sunflower genotypes under salinity stress. The lowest amount of total chlorophyll (۸.۶%) was observed in the salinity level of ۸ dS/m in the sensitive line (C۶۴). Results revealed the C۶۴ and C۶۸ selected genotypes from two our identified sunflower heterotic groups have different physiological response to salinity stress and C۶۸ is more tolerant to salt stress than C۶۴. So, they can be potentially used as parents in sunflower breeding programs to produce salt stress tolerant hybrids.

Keywords:

Antioxidant Activity , Chlorophyll , malondialdehyde , Net photosynthesis , Salinity stress , sunflower