Polymorphisms of renalase gene in Egyptian prevalent hemodialysis patients with and without hypertension

Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JRE-6-1_003

تاریخ نمایه سازی: 30 مرداد 1401

Abstract:

Introduction: Chronic kidney disease (CKD) is considered a very serious worldwide health problem. Renalase is flavin adenine dinucleotide-dependent amine oxidase. It is secreted from the kidney. Renalase release in blood to regulate cardiac function as systemic blood pressure. Relative decrease in renalase enzyme in patients with CKD can be a cause of occurrence of hypertension in CKD patients and an increased risk of cardiovascular complications in end stage renal disease (ESRD) patients.Objectives: In this study, we tried to study the association of the two renalase gene polymorphisms rs۲۲۹۶۵۴۵ and rs۱۰۸۸۷۸۰۰ in ESRD patients and occurrence of hypertension in Egyptian prevalent hemodialysis (HD) patients.Patients and Method: Rs۲۲۹۶۵۴۵ polymorphism and rs۱۰۸۸۷۸۰۰ polymorphism were genotyped in ۳۳۷ subjects that divided into ۲ groups: group I; ۲۵۲ prevalent HD patients that subdivided into two subgroups: Subgroup Ia included ۱۸۷ subjects with hypertension and subgroup Ib included ۶۵ subjects without hypertension. Group II included ۸۵ healthy control subjects. We used the technique of polymerase chain reaction (PCR), followed by cleavage with Eco۸۱ I restriction endonuclease for rs۲۲۹۶۵۴۵ polymorphism and Pst I restriction endonuclease for rs۱۰۸۸۷۸۰۰ polymorphism.Results: Comparison showed that rs۲۲۹۶۵۴۵ CC genotype showed significant increase in HD patients when compared to healthy control and that rs۲۲۹۶۵۴۵ CC genotype showed significant increase in HD patients with hypertension than those without hypertension. In addition, rs۱۰۸۸۷۸۰۰ AA genotype showed significant increase in HD patients when compared to healthy control.Conclusion: Renalase gene polymorphisms might have role in pathophysiology of ESRD especially in hypertensive patients.

Authors

Heba M. Adel Abou Zaghla

Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Tarek M. El-Masry

Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Ramy Salem

Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Mona Abu Zahra

Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt

Mohamed S. Hassan

Internal Medicine Department, Nephrology unit, Faculty of Medicine, Ain Shams University, Cairo, Egypt