Fluorescent Contrast agent Based on Graphene Quantum Dots Decorated Mesoporous Silica Nanoparticles for Detecting and Sorting Cancer Cells
Publish place: Jorjani Biomedicine Journal، Vol: 10، Issue: 3
Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JOBJ-10-3_006
تاریخ نمایه سازی: 10 مهر 1401
Abstract:
Background and Objectives: The inability of classic fluorescence-activated cell sorting to single cancer cell sorting is one of the most significant drawbacks of this method. The sorting of cancer cells in microdroplets significantly influences our ability to analyze cancer cell proteins.
Material and Methods: We adapted a developed microfluidic device as a ۳D in vitro model to sorted MCF-۷ cancer cells on a chip. A prefabricated microfluidic droplet chip was used in this research. Then, with the help of synthesized fluorescent probes, MCF-۷ cancer cells were separated from normal cells.
Results: This research presents a modification of GQD bead for high-throughput analysis and sorting single cancer cells. We elaborate a binding assay as an example of this approach for detecting MCF-۷ cancer cell lines. Graphene quantum dot-decorated mesoporous silica nanoparticles (GQD@MSNPs) act as fluorescent optical beads coated in microfluidic droplets. The fluorescent beads capture cancer cells. To enable droplet sorting at ۲۰۰ Hz and cell enrichment, a measurable fluorescence signal is generated when cancer cells bind to these beads and boost the drop's fluorescence emission.
Conclusion: Herein, we report in vitro results showing that the as-prepared GQD@MSNs have exceptional luminous characteristics. The specific surface area and pore volume of GQD-MSNs were found to be ۵۰% and ۴۰% higher than those of pure MSNs, which is rather remarkable. Because of these improved qualities, GQD@MSNs are demonstrated a large sorting capacity that makes them ideal for diagnosis.
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Authors
Arash Ramedani
Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran, Iran
Abdolreza Simchi
Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran, Iran/ Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran
Omid Sabzevari
Toxicology and Poisoning Research Centre, Tehran University of Medical Sciences, Tehran, Iran/ Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
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