Green synthesis of glucose-coated gold nanoparticles for improving radiosensitivity in human U۸۷ glioblastoma cell line

Objective(s): Surgery and radiation therapy are the most important known treatments for glioblastoma, which is known as the most malignant tumor of the central nervous system. Numerous studies have proven the effect of different gold nanoparticles in improving radiation sensitivity. But there is still a need for nanoparticles with suitable size and higher sensitivity. Hence, the present study aimed to prepare optimized glucose-coated gold nanoparticles (Glu-GNPs) for improving radiosensitivity against U۸۷ glioblastoma cells.Materials and Methods: Firstly, Glu-GNPs were synthesized and then their physiochemical characterizations were assessed using dynamic light scattering (DLS). The cytotoxicity of Glu-GNPs was evaluated by MTT assay in U۸۷ and NIH-۳T۳ cell lines. Additionally, the colony formation assay, which is known as the gold standard test, was used to evaluate the radiosensitivity effect of Glu-GNPs on U۸۷ cells.Results: The characterization results showed that Glu-GNPs had a size of ۵۰.۳ nm and negative zeta potential of -۱۳.۸ mV. Cytotoxicity results revealed that treatment with Glu-GNPs significantly inhibited the proliferation of U۸۷ cells. We found that Glu-GNPs at a concentration of ۱۰ μg/ml were not-toxic for U۸۷ cells. Moreover, the colony formation assay results showed that Glu-GNPs significantly increased the effect of radiation and caused U۸۷ cancer cell death at a non-toxic concentration of ۱۰ μg/ml.Conclusion: Taken together, the Glu-GNPs, with a size of ۵۰.۳ nm, increased radiosensitivity and caused cell death at a concentration of ۱۰ µg/ml in U۸۷ glioblastoma cells and deserve further in vitro and in vivo investigations.

Keywords:

β-D-glucose , Gold Nanoparticle , Radiosensitizer , U۸۷ glioblastoma cell