Evaluation of a functional polymorphism in has-miRNA-۱۹۶a۲ (rs۱۱۶۱۴۹۱۳ C/T) with breast cancer risk in west Iranian women

Backgrounds: miRNAs are non-coding RNAs that play a role in the gene regulation ofoncogenes and tumor suppressors. Single nucleotide polymorphisms (SNPs) in miRNA genes areassociated with the risk of cancer. Some of these SNPs are more common including rs۱۱۶۱۴۹۱۳in the miR-۱۹۶a۲. We aimed to investigate the association between these polymorphisms and therisk of breast cancer in a west Iranian population.Materials and Methods: In this a case-control study enrolled ۱۰۰ patients with sporadic breastcancer and ۱۰۰ health controls incidence matched by age and geographical region. Wegenotyped the functional polymorphism rs۱۱۶۱۴۹۱۳ (C>T) in mir-۱۹۶a۲ by PCR–RFLP andevaluated its relationship with the risk of breast cancer. A ۱۲۳ bp fragment was amplified andthen RLFP assay was performed with MvaI restriction enzymes. The PCR products and digestedfragments were analyzed on ۸% polyacrylamide gel electrophoresis. Allele and genotypefrequencies of SNPs were determined and calculated P-value and risk of disease.Results: Chi-square analysis show a correlation between rs۱۱۶۱۴۹۱۳ (C>T) polymorphism withbreast cancer (p = ۰.۰۱۵, OR= ۱.۸۴; ۹۵% CI: ۱.۱۵-۲.۹۴). The frequency of T alleles for variationwas ۳۵.۵% in cancer patients and ۲۲.۷% controls (P=۰.۰۱). We found that the CT genotype isassociated with the risk of breast cancer (P = ۰.۰۴). In addition, the TT genotype increases therisk of breast cancer, while the comparison of the CC with the CT and TT indicated nosignificant association with the risk for breast cancer. No associations were found betweenpolymorphism and BMI and age.Conclusion: This study indicates that rs۱۱۶۱۴۹۱۳ (C>T) in mir-۱۹۶a۲ polymorphism seems toaffect breast cancer risk and is likely involved in breast cancer susceptibility in addition toenvironmental factors, making its potentially useful genetic biomarkers for disorder screening.

Keywords:

breast cancer , mir-۱۹۶a۲ , polymorphism , RFLP