Kisspeptin is a key component of reproduction that can directly affect food intake in mammals. There is evidence suggesting that melanocortin, GABA, corticotrophin, and neuropeptide Y (NPY), have a mediatory role in reward; however, how these substances interact with kisspeptin-induced by food intake in birds, remains to be identified. Accordingly, in this study, a total of ۱۰ experiments were carried out to investigate the interplay between
kisspeptin and these systems for the control of food intake in neonatal layer-type chickens. In the first experiment, chickens were intracerebroventricular (ICV) injected with saline and Metastin (Kisspeptin, ۰.۲۵, ۵۰, and ۱ nmol). In the second experiment, saline, Metastin (۱ nmol), BIBP-۳۲۲۶ (NPY۱ receptor antagonist, ۱.۲۵ nmol), and co-injection of Metastin + BIBP-۳۲۲۶ were injected. Experiments ۳-۱۰ were similar to experiment ۱, except that chickens received BIIE ۰۲۴۶ (NPY۲ receptor antagonist, ۱.۲۵ nmol), CGP۷۱۶۸۳A (NPY۵ receptor antagonist, ۵۰ μg), Picrotoxin (GABAA receptor antagonist, ۱.۲۵ nmol), CGP۵۴۶۲۶ (GABAB receptor antagonist, ۲۱ µg), astressin-B (CRF۱ / CRF۲ receptor antagonist, ۳۰ µg), Astressin۲-B (CRF۲ receptor antagonist, ۳۰ µg), SHU۹۱۱۹ (MC۳ / MC۴ receptor antagonist, ۰.۵ nmol), and MCL۰۰۲۰ (MC۳ / MC۴ receptor antagonist, ۰.۵ nmol) instead of BIBP-۳۲۲۶. Food intake was subsequently assessed until ۱۲۰ min after the injection. Based on the findings, Metastin (۰.۲۵, ۵۰, and ۱ nmol) significantly increased food intake in a dose-dependent manner (p < ۰.۰۵). However, BIBP-۳۲۲۶ and Picrotoxin inhibited Metastin-induced hyperphagia in neonatal chickens (p < ۰.۰۵); Whereas, whereas BIIE ۰۲۴۶, CGP۷۱۶۸۳A, CGP۵۴۶۲۶, astressin-B, astressin۲-B, SHU۹۱۱۹, and MCL۰۰۲۰ had no effect (p > ۰.۰۵). These results showed that the effect of
kisspeptin on food intake might be mediated by NPY۱ and GABAA receptors in layer-type chickens.