Isolation, morphological and molecular identification of Dunaliella species in Hoz-e Soltan and Aran-va-Bidgol salt Lakes (Iran)

In this study, Dunaliella has been isolated in Jun. ۲۰۱۴ from Hoz-e Soltan and Aran-va-Bidgol salt Lakes (Iran). The isolates have been identified morphologically with the aid of simple light and invert microscope after isolation and purification on their specific medium (Moh۲۰۲). By using molecular techniques such as polymerase chain reaction (PCR) and amplification of ۱۸S rDNA gene primers, further identification was done and morphological identification were confirmed. The isolation and molecular identification of the Dunaliella species from above-mentioned lakes revealed that, at least two species of Dunaliella, namely, D. parva and D. viridis exist in the studied area.

Keywords:

Dunaliella , green algae , molecular diversity , saline lake