Some new species of Mucorales for mycoflora of Iran

In order to determine soil inhabitant fungi of Mazandaran and Golestan provinces (N Iran), some soil samples from arable and non-arable areas were collected. After the isolation, purification and identification of fungi, ۶۱ isolates, belonging to Absidia, Actinomucor, Cunninghamella, Lichtheimia, Mucor, Syncephalastrum and Rhizopus were identified. After DNA extraction, all genera were subjected to PCR using ITS۴ and ITS۵ primers then amplified regions were sequenced. To confirm the morphological identification and sequencing, dendrogram was drawn using MEGA۵ by Maximum Parsimony and Neighbor Joining methods. Dendrograms derived from the primers ITS ۴ & ۵ were separated into three groups. Between the studied isolates, Absidia repens, Cunninghamella blakesleeana and Mucor fragilis (species) and Syncephalastrum racemosum,Lichtheimia ramosa (genera and species) are new records from Iranian mycobiota.