Prevalence of CTX-M, OXA and KPC genes in Klebsiella pneumoniae isolates obtained from patients

Introduction:Klebsiella pneumoniae is known as on e of the most important factors in the development ofopportunistic infections. The main problem in the treatment of infections caused by these organisms is theemergence of strains with multiple resistance, which often leads to prolonged hospital stays, i ncreasedmortality and mobility, increased treatment costs compared to antibiotic sensitive microbes, and ultimatelytreatment failure. Therefore, the aim of this study was to investigate the prevalence of CTX M , OXA and KPCgenes in Klebsiella pneumoniae isolates obtained from patients.Materials and Methods: In this study, ۶۳ isolates of Klebsiella pneumoniae were obtained from diff erentclinical specimens. After final diagnosis of the strains using standard biochemical and microbiologicalmethods, cellular DNA was obtained using Cinaclon's DNA extraction kit. Finally, multiplex PCR test wasperformed to evaluate the presence of OXA ۴۸, CTX M and KPC genes in Eppendorf device using a pair ofspecific primers.Results:Out of ۶۳ samples under study, ۲۹ samples (۴۶%) from urine, ۱۵ samples (۲۳.۸%) from sputum, ۹samples (۴۱.۳%) from fecal samples, ۵ samples (۷.۹%) from w ound culture and ۴ samples (۶.۳%) wereobtained from intravascular catheter of blood culture and ۱ (۱.۶%) sample was obtained from cerebrospinalfluid. The results of PCR test for the studied genes showed that ۴۹ (۷۷.۸%), ۴۹ (۷۷.۸%) and ۴۶ (۷۳%) strainscarried OXA , KPC and CTX M genes, respectively.Conclusion:The results of this study indicate that the frequency of resistance genes in Klebsiella pneumoniaestrain is high and these strains can transfer resistance genes with high potential to other strains. Therefore,detection of Klebsiella pneumoniae strains containing beta lactamase resistance enzymes is important forbetter treatment and prevention of the spread of these genes to other bacteria u sing accurate phenotypic andgenotypic methods.

Keywords:

Klebsiella pneumoniae , A ntibiotic resistance , PCR