Effects of Storage time and Antioxidants on Sperm DNA Quality for different time periods at ۴–۶°C.

Publish Year: 1401
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:

WMCONF07_028

تاریخ نمایه سازی: 14 آذر 1401

Abstract:

Background: In infertility clinics, preserving high-quality spermatozoa for a long time is a necessity. Pentoxifylline (PT) and L-carnitine (LC) are two effective antioxidants in improving sperm quality. The present study aimed to evaluate the effects of Pentoxifylline (PT) and L-carnitine (LC on the quality of sperm DNA, and protamine content on prolonge storage for۱۲ days at ۴-۶°C.Materials and methods: The present experimental work included ۲۶ samples of normozoospermic men. After swim up, samples were divided into three aliquots as untreated control, LC-treated, and PT-treated groups and incubated for up to ۱۲ days at ۴-۶ °C. Thereafter, DNA denaturation and chromatin maturity were assessed on ۰, ۱, ۲, ۷, and ۱۲ days. Data were analyzed using a one-way analysis of variance.Results: The obtained data revealed that during the ۱۲-day storage, the percentage of spermatozoa with double stranded DNA and the percentage of spermatozoa with a normal protamine content was nearly unchanged in the three groups (p-value >۰.۰۵).Conclusion: Sperm DNA was resistant against changes during ۱۲ days at ۴-۶ °C in all groups

Authors

Zohre Nateghian

Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Elham Aliabadi

Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran