Neobaicalein, a flavonoid from the Scutellaria litwinowii Bornm. & Sint. ex Bornm. induced apoptosis in human leukemic cell lines

Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJBMS-26-3_002

تاریخ نمایه سازی: 8 بهمن 1401

Abstract:

Objective(s): Neobaicalein is one of the rich plant flavonoids isolated from the roots of Scutellaria spp. In this study, we evaluated and compared cytotoxic activity and the related apoptosis mechanisms of neobaicalein from Scutellaria litwinowii Bornm. & Sint. ex Bornm on apoptosis-proficient HL-۶۰ cells and apoptosis-resistant K۵۶۲ cells. Materials and Methods: Cell viability, cell apoptosis, caspase activity, and apoptosis-related protein expression were measured using MTS assay, propidium iodide (PI) staining and flow cytometry, caspase activity assay, and western blot analysis, respectively. Results: Neobaicalein significantly reduced cell viability in a dose-dependent manner using the MTS assay (P<۰.۰۵). The IC۵۰ values (µM) against HL-۶۰ and K۵۶۲ cells after ۴۸ hr treatment were ۴۰.۵ and ۸۴.۸, respectively. Incubation of HL-۶۰ and K۵۶۲ cells with ۲۵, ۵۰, and ۱۰۰ µM neobaicalein for ۴۸ hr, significantly increased the number of apoptotic cells and showed cytotoxic effects compared with the control group. Treatment with neobaicalein significantly increased Fas (P<۰.۰۵) and the cleaved form of PARP (P<۰.۰۵), and decreased the Bcl-۲ levels (P<۰.۰۵) in HL-۶۰ cells, whereas neobaicalein significantly increased Bax (P<۰.۰۵) and the cleaved form of PARP (P<۰.۰۵), and the caspases of the extrinsic and intrinsic pathways including caspases-۸ (P<۰.۰۰۰۱), -۹ (P<۰.۰۱), and effector caspase-۳ (P<۰.۰۰۰۱) levels in K۵۶۲ cells compared with the control group.Conclusion: It seems neobaicalein might cause cytotoxicity and cell apoptosis through interaction with the different apoptosis-related proteins of apoptotic pathways in HL-۶۰ and K۵۶۲ cells. Neobaicalein may exert a beneficial protective effect in slowing the progression of hematological malignancies.

Authors

Seyed Ahmad Emami

Department of Traditional Pharmacy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Elham Ramazani

Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

Seyed Hadi Mousavi

Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Nasser Vahdati-Mashhadian

Medical Toxicology Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran

Javad Asili

Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Heidar Parsaee

Department of Pharmacology and Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Zahra Tayarani-Najaran

Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran

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