Evaluating the Distribution of (+ ۲۰۴۴G / A, R۱۳۰Q) Rs۲۰۵۴۱ and (-۱۱۱۲ C / T) Rs۱۸۰۰۹۲۵ Polymorphism in IL-۱۳ Gene: An Association-Based Study with Asthma in Ahvaz, Iran

Background and Aims: Asthma is a complicated chronic inflammatory disease associated with pulmonary inflammation, severe immune responses of the respiratory tract, and change or destruction in the respiratory tract structure. Several factors, especially genetic and environmental factors, contribute to the pathogenesis of asthma. Interlukin (IL)-۱۳ is considered the most important inflammatory mediator of asthma that play an effective role in the different stages of B cell maturation and differentiation. It also increases the expression of major histocompatibility complex II and CD۲۳ and is effective in IgE isotypes switching. Single nucleotide polymorphisms (SNPs) cause the wide genetic diversity in the genome, and SNP analysis helps evaluate and diagnose disease-related genes. The present study was conducted to investigate the association of -۱۱۱۲C / T and + ۲۰۴۴ G /A polymorphisms in the IL-۱۳ gene with asthma. Material and Methods: Blood samples (۵ ml) from asthma patients (۱۶۷) and controls (۱۷۲) after spirometry test were collected into tubes, and then DNA was extracted to determine the genotype of asthma patients. Real-time polymerase chain reaction was performed by the Taq Man method, and the results were analyzed using SPSS software version ۲۲. Results: There was a significant difference between the genotype of IL-۱۳ -۱۱۱۲C / T polymorphism in the patient group and the control group (p = ۰.۰۲۸). The IL۱۳ + ۲۰۴۴G / A polymorphism results showed no significant difference between the two groups (p = ۰.۳۱۹). Conclusions: The present study showed that IL-۱۳ -۱۱۱۲C / T polymorphism positively correlates with the induction of asthma, but there was no significant association between the polymorphism + ۲۰۴۴G / A IL-۱۳ and the risk of asthma.

Keywords:

Asthma , IL-۱۳ , polymorphism , Real time PCR , -