سیویلیکا را در شبکه های اجتماعی دنبال نمایید.

The Effect of Incubation Time on the Activity and Stability of Factor VIII during the Preparation Process

Publish Year: 1398
Type: Journal paper
Language: English
View: 183

This Paper With 8 Page And PDF Format Ready To Download

Export:

Link to this Paper:

Document National Code:

JR_JIML-6-3_006

Index date: 25 February 2023

The Effect of Incubation Time on the Activity and Stability of Factor VIII during the Preparation Process abstract

Background and Aims: Hemophilia is a rare autoimmune disorder caused by autoantibodies directed in the majority of the cases against clotting factor VIII (FVIII). FVIII is extracted from human plasma or engineered from mammalian cell cultures using recombinant DNA technology. In Iran, most of the used FVIII is prepared from human plasma in Iranian Blood Transfusion Organization. It was seen important to estimate its stability and activity from bleeding time until product preparation. Material and Methods: In the analytical study, 60 healthy male donors (20-50 years old), 15 donors from each blood group, were selected after obtaining informed consent. Donors' blood was collected in QUADRI-PACKs and centrifuged after keeping at 24°C for 2 hours. The separated plasma was divided into three groups and incubated in the lab (22°C) for 0, 90, and 180 minutes, respectively. Then, samples were stored at -20°C for one month. Afterward, the plasma was thawed, and FVIII activity was assayed. Results: The activity of FVIII significantly (p<0.05) reduced by delay in freezing; after the time of 0 min: 134.84%±42, after 90 min: 126.88%±38, after 180 min: 120.22%±34. At all incubation times, the highest and the lowest FVIII activity were observed in A and O blood groups, respectively (p<0.05). FVIII activity was increased along with increasing age up to 35-40, but it decreased in subjects of 40-50 years old. These experiments confirmed that the longer the delay in freezing fresh frozen plasma, the greater the decrease in FVIII stability. Conclusions:. According to the results of this study, the best blood donors for FVIII product are those with blood group A in the age range of 40-35 years.

The Effect of Incubation Time on the Activity and Stability of Factor VIII during the Preparation Process Keywords:

The Effect of Incubation Time on the Activity and Stability of Factor VIII during the Preparation Process authors

کوروش کبیر

Social Determinants of Health Research Center, Alborz University of Medical Sciences, Karaj, Iran.

حسن حسینی

Blood Transfusion Researcher Center, Institute for Higher Education and Research in Transfusion Medicine, Tehran, Iran.

مهدی جاهد زرگر

Department of Hematology, Faculty of Paramedical, Alborz University of Medical Sciences, Karaj, Iran.

زینب مانده

Blood Transfusion Researcher Center, Institute for Higher Education and Research in Transfusion Medicine, Tehran, Iran.

فاطمه امرالهی

Blood Transfusion Researcher Center, Institute for Higher Education and Research in Transfusion Medicine, Tehran, Iran.

نوید فرهمندیان

Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.

الهام بحرینی

Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
. Wakabayashi H, Freas J, Zhou Q, Fay PJ. Residues ...
. Fay PJ. Activation of factor VIII and mechanisms of ...
. Lusher JM, Milestones in hemophilia and concepts in future ...
. Fijnvandraat K, Peters M, Ten Cate JW. Inter‐individual variation ...
. Franchini M, Mannucci PM. Past, present and future of ...
. Lynch CM, Israel DI, Kaufman RJ, Miller AD. Sequences ...
. Lusher JM, Arkin S, Abildgaard CF, Schwartz RS. Recombinant ...
. Dasgupta S, Repessé Y, Bayry J, Navarrete AM, Wootla ...
. Scharrer I, Bray G, Neutzling O. Incidence of inhibitors ...
. Mannucci PM, Chediak J, Hanna W, Byrnes J, Ledford ...
. Pool JG, Shannon AE. Production of high-potency concentrates of ...
. Rodgers SE, Duncan EM, Barbulescu DM, Quinn DM, Lloyd ...
. Carlebjörk G, Oswaldsson U, Rosén S. A simple and ...
. Smith JF, Ness PM, Moroff G, Luban NL. Retention ...
. Swärd‐Nilsson AM, Persson PO, Johnson U, Lethagen S. Factors ...
. Franchini M, Capra F, Targher G, Montagnana M, Lippi ...
. Gallinaro L, Cattini MG, Sztukowska M, Padrini R, Sartorello ...
. Gill JC, Endres-Brooks J, Bauer PJ, Marks WJ, Montgomery ...
. O'donnell J, Laffan M. The relationship between ABO histo‐blood ...
. Song J, Chen F, Campos M, Bolgiano D, Houck ...
. Smith NL, Chen MH, Dehghan A, Strachan DP, Basu ...
. Wang Z, Dou M, Du X, Ma L, Sun ...
. Cohen W, Castelli C, Alessi MC, Aillaud MF, Bouvet ...
. Gonskikh Y, Polacek N. Alterations of the translation apparatus ...
نمایش کامل مراجع