Research Article: Efficiency of monovalent Vibrio alginolyticus formaldehyde-killed vaccine on the immune responses and protection of Asian seabass (Lates calcarifer) juveniles against Vibriosis

Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
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JR_JIFRO-21-6_002

تاریخ نمایه سازی: 10 اسفند 1401

Abstract:

Vibrio alginolyticus causes severe health problems in marine fish production each year. Among various therapeutic strategies, vaccination is the most economic, efficient and environmentally-friendly approach against microbial infections. Evaluation of formalin-killed Vibrio alginolyticus vaccine aquired from native isolates was carried out using a total of ۲۰۰ Asian seabass juveniles (۵۵±۶.۴۳ g), which were divided among three groups, including (I) control (non-vaccinated), (II) vaccinated with killed V. alginolyticus and (III) killed vaccine with oral booster. Fish were vaccinated intraperitoneally. Blood samples were taken from fish in each group at three, five, and eight weeks after immunization to assess the antibody levels against V. alginolyticus infection. The efficacy of the killed vaccine was appraised five weeks after the start of the initial vaccination by challenging with twofold LD۵۰ (۳.۶۶×۱۰۸) equivalent of the live suspension of V. alginolyticus through intraperitoneal injection. The results of ELISA showed that there were not any significant differences in antibody response among different groups before vaccination (p>۰.۰۵). Also, the mean antibody titer of the group immunized killed-V. alginolyticus with oral booster was significantly higher than the other groups five weeks after the vaccination. The vaccined fish demonstrated higher survival rates than the control with relative percent survival (RPS) of ۸۴.۶۲ and ۷۶.۹۲%, respectively. It is concluded that vaccination could be an effective method to protect farmed Asian sea bass against vibriosis caused by pathogenic V. alginolyticus.

Authors

M. Ahangarzadeh

South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran.

H. Houshmand

South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran.

S. Kakoolaki

Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran.

A. Sepahdari

Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran.

M. Ghorbanpoor

Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

A. Ajdari

Offshore Fisheries Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Chabahar, Iran.

S. Nazemroaya

South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran.

M. Zabayeh Najafabadi

South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran.

M. Torfi Mozanzadeh

South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran.

A.S. Sadr

South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran.

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