In Vitro Production of Bulblet in Galanthus transcaucasicus Fomin, an Endangered Medicinal Plant
Publish place: Journal of Plant Physiology and Breeding، Vol: 6، Issue: 2
Publish Year: 1395
Type: Journal paper
Language: English
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Document National Code:
JR_BREDNG-6-2_001
Index date: 5 March 2023
In Vitro Production of Bulblet in Galanthus transcaucasicus Fomin, an Endangered Medicinal Plant abstract
To optimize conditions for micropropagating Galanthus transcaucasicus Fomin, the effect of explant type, different concentrations of sucrose, type and different concentrations of auxin were examined on bulblet production. For the induction of bulblets, three types of bulb scales were usesd as explants. A two-step sterilization procedure was applied. Assessments were made after incubation for 20 weeks at 23±1ºC with 16h photoperiods. Highest number of bulblets were proliferated culturing bulb scales on modified MS medium supplemented with 2.0 mg.l-1 BA and 2.0 mg.l-1 IBA. About 27% of bulblets were bigger than 5 mm in diameter, suitable for acclimatization. With the increase of sucrose concentration, number of bulblets decreased, but their size increased. This protocol provided a basis for future study on large-scale multiplication system for commercial nurseries of Galanthus transcaucasicus.
In Vitro Production of Bulblet in Galanthus transcaucasicus Fomin, an Endangered Medicinal Plant Keywords:
In Vitro Production of Bulblet in Galanthus transcaucasicus Fomin, an Endangered Medicinal Plant authors
Marzieh Babashpour-Asl
Department of Horticultural Science, Maragheh Branch, Islamic Azad University, Maragheh, Iran
Ali Movafeghi
Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
Khadijeh Zare
Department of Biology, Ahar Branch, Islamic Azad University, Ahar, Iran
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