Study of Release Behavior of Carboplatin from Modified Immunoglobulin Nanoparticles by Folic Acid: Preparation, Characterization and Analytical Approaches

The sustained release potential of bioactive materials and drugs is a major requirement in the development of carriers for cancer treatment. In this study, Carboplatin (Crb) as a standard anticancer drug was loaded to immunoglobulin G nanoparticles (IgGNPs) in the absence (Crb@IgGNPs) and the presence of folic acid (FA), ([e-mail]) as a targetable agent. Their physicochemical properties were characterized by various techniques. Dynamic light scattering (DLS) technique indicated that the average hydrodynamic diameter and zeta potential values of Crb@IgGNPs and [e-mail] were 831.23±4.95 nm; (PDI: 0.980.31), 397.47±22.96 nm; (PDI: 0.780.08) and -2.97±1.17 mV, -7.06±0.72 mV, respectively. The spherical shapes of the nanocarriers showed more particle size distribution in [e-mail] are confirmed by field emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM). Fourier-transform infrared (FTIR) spectra of nanocarriers confirmed Crb loading onto IgGNPs and FA.IgGNPs. Afterwards, In vitro release study of Crb and [e-mail] was performed that demonstrated Crb was slowly released from FA.IgGNPs (about 61 h longer than only Crb) and the release mechanism was followed by korsmeyer-peppas model with Fickian diffusion. Overall, it was observed that the novel designed drug carrier improved the drug release with the appropriate properties for clinical approaches.

Study of Release Behavior of Carboplatin from Modified Immunoglobulin Nanoparticles by Folic Acid: Preparation, Characterization and Analytical Approaches Keywords:

IgG , Nanocarrier , Carboplatin , Folate , release