The Antibiotic Resistance Pattern and Prevalence of blaTEM, blaSHV, blaCTX-M, blaPSE-۱, sipB/C, and cmlA/tetR Genes in Salmonella typhimurium Isolated from Children with Diarrhea in Tabriz, Iran
Publish place: Journal of Health Reports and Technology، Vol: 7، Issue: 4
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
View: 119
This Paper With 7 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJHLS-7-4_007
تاریخ نمایه سازی: 28 تیر 1402
Abstract:
Background: Salmonella gastroenteritis is a global health concern. Recently, increased resistance to Salmonella typhimurium has
been reported in several countries.
Objectives: The present study aimed to evaluate the prevalence of blaTEM, blaSHV, blaCTX-M, blaPSE-۱, sipB/C, and cmlA/tetR genes in S.
typhimurium isolates and determine their antibiotic resistance.
Methods: This cross-sectional descriptive study was conducted on ۱۱۰ fecal samples, which were collected from the patients referred
to the hospitals and medical centers in Tabriz, Iran during eight months. After phenotypic identification, the antibiogram test and
double-disc synergy test were performed on the isolates. Following that, the prevalence of resistance genes was evaluated using
multiplex PCR and specific primers.
Results: Out of ۱۱۰ fecal samples, ۲۶ samples (۲۳.۶۳%) were positive for S. typhimurium. The highest resistance of the isolates was
against ceftazidime, cefotaxime, amikacin, and tetracycline (۱۰۰%), and the lowest resistance was against imipenem (۳.۸۵%) and
nalidixic acid (۷.۶۹%). In total, ۱۵ S. typhimurium isolates (۵۷.۶۹%) were positive for the extended-spectrum beta-lactamases. In addition,
the most common resistance genes in the isolates were cmlA/tetR (۳۸.۴۶%), blaTEM (۳۴.۶۱%), and blaCTX-M (۲۶.۹۲%). Four isolates
(۱۵.۳۸%) carried sipB, three isolates (۱۱.۵۳%) contained blaSHV, and two isolates (۷.۶۹%) carried blaPSE-۱.
Conclusions: The obtained results indicated the high prevalence of antibiotic-resistant S. typhimurium. Therefore, the identification
of resistance genes is an important strategy for identifying and counteracting antibiotic resistance.
Authors
Abolfazl Jafari Sales
Department of Microbiology School of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran
Sara Naebi
Department of Microbiology School of Basic Sciences, Ahar Branch, Islamic Azad University, Ahar, Iran
Rozita Nasiri
Iran National Elite Foundation, ۹۳۱۱۱-۱۴۵۷۸ Tehran, Iran
Hossein Bannazadeh-Baghi
Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran