Biofilm Formation of Salmonella typhimurium on Stainless Steel in Red Meat Model and the Effect of Bacteriophage on Bacterial Biofilm

Background and Aims: The application of bacteriophage to control and removal of bacterial biofilm is a novel method. The goal of this study was to investigate the effect of the Salmonella typhimurium bacteriophage against biofilm of a multidrug resistance (MDR) Salmonella formed on stainless steel in beef broth. Materials and Methods: One and ۷ days old biofilm were grown at ۱۵, ۸ and ۴ °C, on the stainless steel in beef broth and the effects of different bacteriophage concentrations (۱۰۳, ۱۰۵ and ۱۰۷ PFU/mL) with two contact times (۱۰ and ۱۵ min) were assayed. Results: Results showed that Salmonella can adhere to stainless steel and form biofilm in the beef broth which was significantly influenced by temperature. Higher biomass of biofilm was developed at ۱۵, ۴ and ۸ °C, respectively. One-day-old is less dense (~۱ logarithmic cycle) than ۷-day-old biofilm. No significant difference (P>۰.۰۵) in biofilm reduction was observed in samples treated with different concentration as compared with control. Statistical differences were also not observed in the different contact time (۱۰ and ۱۵ min). Conclusions: These results indicated that there was no significant reduction in MDR Salmonella biofilm population developed on stainless steel in the beef broth after using the bacteriophage; it is needed to investigate some combination procedures or increase contact time to improve the biofilm removal activity of bacteriophage.

Keywords:

Bacteriophage , Salmonella typhimurium , Biofilm , Red meat , باکتریوفاژ , سالمونلا تیفی موریوم , بیوفیلم , گوشت قرمز