The Identification of pathogenic Escherichia coli strains to carry intimin gene isolated from various water sources

Background and Aim: Escherichia coli  is considered  as  one  of  the  most  important  causes  of   bacterial    infections   transmitted   through   water   and  foods. Several factors are involved in the pathogenesis of Escherichia coli  .  The aim  of  this  study  was  to   investigate  the  prevalence of Escherichia coli  strains carrying  virulence gene  intimin isolated from different water sources in  Alborz province. Materials and Methods: This study was carried  out in ۲۰۱۳.   The  research  included   all  E. coli  strains isolated from different surface water sources in Alborz province. Escherichia coli isolates were detected and identified by standard microbiological and biochemical tests. The strains were evaluated for the presence of intimin gene by  PCR using specific primers. Results: One hundred E. coli  strains  were isolated  then PCR was successfully optimized for detection of intimin gene. The  PCR  results  showed  ۳۳%  of  the  strains carried out  intimin  gene. Conclusions: Compared to many studies, our findings showed the high frequency of E. coli  strains carrying  intimin gene  isolated from surface water sources. The presence of this virulence genes is a potential threat for contamination of drinking water which is important for health care systems.

Keywords:

Escherichia coli , PCR , intimin gene. , اشریشیا کلی , PCR , ژن اینتیمین