Evaluation of a New Colorimetric Medium for Detection of Extended Spectrum β-lactamases (ESBLs) Producing Isolates of Entrobacteriaceae family
Publish place: Iranian Journal of Medical Microbiology، Vol: 10، Issue: 2
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
View: 52
This Paper With 8 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJMM-10-2_008
تاریخ نمایه سازی: 16 مرداد 1402
Abstract:
Background and Aim: Due to the increased growth of bacteria producing ESBL, among the members of the Enterobacteriaceae family, monitoring and treatment of nosocomial infections are important, especially caused by ESBL-producing clinical isolates belonging to this family. The aim of this study is to evaluate a rapid method, using new colorimetric culture was made at the University of Al-Zahra (Tehran, Iran).The used method to detect ESBLs, according to disk diffusion method based on CLSI on colorimetric culture and the results of it, compared with the Müller-Hinton agar for disk diffusion method.
Materials and Methods: In this study, the clinically isolated samples belonged to the Enterobacteriaceae family which was evaluated in both Müller-Hinton agar medium and colorimetric culture using ceftazidime (CAZ), ceftazidime + clavulanate (CZA), cefotaxime + clavulanate (CTC) and Cefotaxime (CTX) discs.
Results & Conclusions: Because of the metabolic activity of bacteria, a color change occurred from red to yellow in colorimetric culture after ۵-۶ hours and red inhibitory zone created around the disc. The obtained results from the colorimetric method are same with the results of Müller-Hinton agar method for disk diffusion after a day.
Keywords:
Authors
Shirin Shahsavari
Biotechnology Department, Faculty of Biology Sciences, Alzahra University, Tehran, Iran
Siavosh Salmanzade Ahrabi
Microiology Department, Faculty of Sciences, Alzahra University, Tehran, Iran
Tahereh Falsafi
Microiology Department, Faculty of Sciences, Alzahra University, Tehran, Iran
Gholam Reza Irajian
Medical Microbiology Department, Iran University of Medical Sciences, Tehran, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :