The Association Between Higher Expression of Talin-۱ and the Reduced Survival Rate in Ovarian Serous Carcinoma Patients

Background & Objective: Talin-۱ is a constituent of the multiprotein adhesion complexes that play  main role in the formation of tumors and migration in different types of malignancies. The present study aimed to assess  expression and prognostic significance of the talin-۱ protein in ovarian serous carcinoma (OSC) patients.Methods: The expression of talin-۱ in mRNA and its protein levels were investigated for ovarian cancer (OC) by using bioinformatics tools, including Gene Expression Profiling Interactive Analysis ۲ (GEPIA۲), Gene Expression Database of Normal and Tumor Tissue ۲ (GENT۲), and The University of ALabama at Birmingham CANcer data analysis Portal (UALCAN) databases. Thereafter, immunohistochemical (IHC) staining was used to study the expression patterns of the talin-۱ protein using ۴۶ paraffin-embedded OSC tissue specimens, ۲۵ benign tumors, and ۲۰ normal tissues, which were assembled in tissue microarrays (TMAs). We also assessed the potential association between the expression of the talin-۱ protein, various clinicopathological parameters, and survival outcomes.Results: Our IHC examination for talin-۱ was significantly overexpressed in OSC tissues compared to benign tumors and normal tissues. The Kaplan-Meier survival analysis has also indicated statistically significant differences in terms of disease-specific survival (DSS) and progression-free survival (PFS) between the patients with high and low expression levels of talin-۱, respectively.Conclusion: The talin-۱ protein was overexpressed in OSC tissues, and a high expression level of talin-۱ was found to be significantly associated with tumor aggressiveness and poorer DSS or PFS. Therefore, talin-۱ may serve as a molecular marker of cancer progression and a novel prognostic biomarker in these patients.

Keywords:

Cancer progression , Ovarian serous carcinoma , Prognosis , Talin-۱ , Tissue microarray