Evaluation of Circulating Leptin and Its Receptor (Ob-R) Tissue Expression in Colorectal Cancer, a Report From North of Iran

Background & Objective: Leptin is an adipocyte-derived hormone with a critical role in energy balance. As demonstrated by previous investigations, leptin acts as a proliferative and angiogenic factor in cancer cells. However, results regarding its role in colorectal cancer are still inconclusive. We aimed to evaluate serum leptin and tissue expression of leptin receptor (Ob-R) in normal and malignant samples of colorectal.Methods: Serum and tissue samples from pathology-confirmed colorectal cancer patients and normal controls referring to a university hospital of Mazandaran were obtained during ۲۰۱۹-۲۱. ELISA and immunohistochemistry were applied to determine leptin and Ob-R expression respectively.Results: A total of ۹۰ samples belonging to ۴۶ normal and ۴۴ CRC patients were enrolled. Normal and CRC groups included ۳۲ (۶۹.۵۶%) and ۲۱ (۴۷.۷۲%) female subjects respectively. The average leptin concentration in the normal group was ۱۱۵.۸۰ and, in the patient, group was ۱۲۴.۴۷ ng/mL (P=۰.۸۹۷). CRC cases showed an insignificantly higher Ob-R detection rate (P=۰.۰۸۶).Conclusion: There was no significant difference in leptin and Ob-R expression between CRC patients and normal subjects. Thus, leptin and its receptor may not be useful as a biomarker of CRC.

Keywords:

Colorectal cancer , Immunohistochemistry , Leptin , Leptin receptor , LEP , LEPR , Phenotype