Mesenchymal Stem Cell Growth Behavior on 2-D Carbonated Hydroxyapatite Scaffolds Prepared by Three Different Methods
Publish place: 1st International and 6th Joint Conference of Iranian Society of Metallurgical Engineers and the scientific community of Casting
Publish Year: 1391
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
IMES06_034
تاریخ نمایه سازی: 8 بهمن 1391
Abstract:
This study aims at the evaluation of new approaches to culture umbilical cord mesenchymal stem cells (UC-MSCs) on 2-dimensional carbonated hydroxyapatite (CHAp) scaffolds extracted from bio-waste of bovine bones femur. To extract CHAp bioceramics, three different treatments have been applied to achieve pure natural CHAp: Calcination heat treatment (at temperature of 700 ℃), alkaline hydrothermal (275 ℃) and pressurized low polarity water (PLPW) (250 ℃). Raw bovine bone and obtained apatites have been characterized by Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction(XRD). The FT-IR spectrums indicated that all of the utilized processes have the ability of plucking collagen and other organic compounds out of bovine bones, and finally leaving pure natural CHAp withyield of ~60%. In these processes, obtained apatites leave carbonate contents in specific temperatures,which leads to increased biological effects in derived CHAp and preferable for orthopedic and medicalusages. Fluorescent Microscopy and Scanning Electron Microscopy (SEM) were used to investigate UCMSCs growth behavior, Viability, and adhesion of Cells on the surface of 2-D Hydroxyapatite Scaffolds derived by these three different methods. The findings suggest that UC-MSCs could survive and attach onto these scaffolds and demonstrate no negative response to carbonated hydroxyapatite
Keywords:
Umbilical Cord Mesenchymal Stem Cell , Carbonated Hydroxyapatites , Bovine bone , Flowcytometery , Physiochemical characterization
Authors
Abbas Fazel Anvari-Yazdi
Research & Development in Materials Engineering, Yazd Science &Technology Park, Yazd, Iran
Mahdi Kalantar
Assistant Professor, Faculty of Materials Science Engineering, Yazd University
Tahereh Talaei-Khozani
Associated Professor, Laboratory for Stem Cell Research, Department ofTissue Engineering, Shiraz University of Medical Sciences (SUMS), Shiraz, Iran
Arash Yazdani
Ph.D. Student, Department of Materials Science Engineering, Faculty of Engineering,Shiraz University, Shiraz, Iran
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