Ellagic acid alleviates motor, cognitive and hippocampal electrical activity deficits in the male rats with ۲-vessel occlusion cerebral ischemia/reperfusion

Objective: Cerebral ischemia/reperfusion (I/R) has been known as a major cause of inability and mortality worldwide. Ellagic acid (EA) has many pharmacological effects including antioxidant, antithrombotic and neurorestoration activities. The aim of this study was evaluation of the effects of EA on motor and cognitive behaviors, hippocampal local field potential (LFP), brain oxidative stress in male rats with cerebral ۲-vessel occlusion ischemia/reperfusion (۲VO I/R).Materials and Methods: Forty-eight male Wistar rats (۲۵۰-۳۰۰ g) were assigned into six groups. ۱) The Sham: rats were treated with DMSO۱۰%/normal saline as solvent of EA ۳ times daily for ۱ week; ۲) I/R+Veh; I/R rats received vehicle; ۳-۵) EA-treated groups: I/R rats received ۵۰, ۷۵, or ۱۰۰ mg/kg EA; and ۶) Cont+EA۱۰۰: intact rats received EA. The cerebral ۲VO I/R was made by the bilateral common carotid arteries closing for ۲۰ min followed by reperfusion. The behavioral tests and hippocampal LFP recording were performed after treatment with EA. The oxidative stress parameters were assayed by special ELISA kits.Results: Cerebral ۲VO I/R significantly decreased motor coordination, memory and hippocampal LFP and significantly increased oxidative stress. Treatment with EA improved all I/R complications. Conclusion: The current findings showed that treatment of I/R rats with EA could reverse cognitive and motor functions, and improve the LFP and oxidative stress markers. So, effects of EA on cognitive and motor function may at least in part, be due to its antioxidative actions.

Keywords:

Cerebral ischemia/reperfusion , Ellagic acid , Motor coordination , Cognition , Hippocampal local EEG , Oxidative stress