Insecticidal activities of some essential oils against larval ectoparasitoid, Habrobracon hebetor (Hymenoptera: Braconidae)

Habrobracon hebetor Say is an idiobiont and gregarious larval ectoparasitoid of many moths. In this study, lethal and sublethal effects of Eucalyptus camaldulensis, Carum carvi and Heracleum persicum essential oils on the demographic parameters of H. hebetor were assessed at ۲۶ ± ۲ °C, ۶۰ ± ۵% RH, and a photoperiod of ۱۶:۸ (L: D) h. Essential oils were obtained from these plants by hydro-distillation method using a Clevenger apparatus. The chemical constituents of essential oils were detected by Gas Chromatography-Mass spectrometry (GC-MS). ۲۵۰ml Glass vials were used for the fumigant toxicity experiments. In order to assess the sublethal effects, adult wasps were exposed to an LC۲۵ of each essential oil and then the demographic parameters of live parasitoid wasps were studied. Fumigant toxicity with adults indicated that the lethal concentration (LC۵۰) values of the above essential oils against H. hebetor females were ۱.۱۱۶, ۰.۳۴ and ۳.۴۱۶μl/l air, respectively. Chemical analysis by GC-MS displayed o-Cymene (۱۵.۱۱%), Carvone (۵۵.۸%) and Hexyl butyrate (۴۱.۷۸%) were main constituents of the essential oils of E. camaldulensis, C. carvi and H. persicum, respectively. The results showed that the intrinsic rate of increase (r), finite rate of increase (λ), net reproductive rate (R۰) and gross reproductive rate (GRR) were significantly affected by the essential oils. The highest and the lowest r values were ۰.۲۲۶ and ۰.۱۳۰ day-۱ in control and C. carvi-treated insects, respectively. According to these results, essential oils have suitable potential for the integrated management of stored product pests.

Keywords:

Essential oils , fumigant toxicity , GC-MS , parasitoid wasp , اسانس های گیاهی , سمیت تدخینی , GC-MS , زنبور پارازیتوئید