Transcriptome analysis of tobacco in response to Ralstonia solanacearum infection

One of the best strategies to control bacterial wilt caused by Ralstonia solanacearum (Smith) is generally based on breeding resistant cultivars. The information obtained from the expression of plant defense genes will provide new insight for improving plant resistance against pathogens. This study was to identify inducible genes under defense no death (DND) reaction of tobacco (Nicotiana tabacum)-R. solanacearum interaction using cDNA-AFLP technique. In this assay five different primer combinations were used. Out of ۱۳۲۰ Transcript derived fragments (TDF) that were detected, ۱۰۱ fragments were identified as differentially expressed genes in ۰, ۲۴, ۴۸ and ۷۲ hours post inoculation. Most of the differentially expressed genes were obtained ۴۸ hours post inoculation. Following sequencing, most of sequenced TDFs showed homology to known genes interfering in signaling, regulation and defense functions. DND phenotype in tobacco has some similarities specially in signaling process with mechanism associated with induction of the hypersensitive reaction and it is distinct from general defense mechanisms.

Keywords:

Tobacco , Ralstonia solanacearum , Transcriptome , Interaction