Serological Detection and Symptomology of TomatoSpotted Wilt Virus in Tehran Province, Iran

ABSTRACT Tomato spotted wilt virus (TSWV) was detected in tomato leaf and fruit samples collected from Viiramin reyion in Tehran province using indicator test plants including Petunia hybrida, Nicatiana glutinosa, N. tabacum cv. Snmsun NN, N. clevelandit and N. benthamiana and seroloyical tests. Small browinsh local necrotic lesions appeared on P. hybrida leaves ۲-۴ days post-inoculation. Systemic symptoms included concentric ring spots on leaves, stem necrosis, wilting and tissue collapse of plants ۷-۱۰ days following the inoculation. Among ۱۴۵ tomato samples collected from Ghazvin, Hashtgerd, Karaj, Malard, Shahriar and Varamin in Tehran province, only Varamin samples were infected with TSWV using EL۱SA, DIBA and SSEM. TSWV host range specificity and symptom expression were tested on Capsicum annuum L., Chenopodium amaranticolor I«, Citrullus vulgaris L., Oicumis melo var. inodorus, C. mela var. reticukUus, C. salivas L., Lycopersicon esculentum Mill., Phasenlus vulgaris L., Solatium melongena ۱- and S. tuberosum. Typical symptoms on these plants included concentric ring spots, chlorosis, vein clearing, tissue necrosis, stunting and local lesion formation. Antiserum prepared against a partially purified TSWV preparation cross-reacted with TSWV-infected tomato samples.

Keywords:

Tomato , Tomato Spotted Wilt Virus , SSEM , DAS-ELISA , DAS-DIBA