Mucilage Production In Tissue Culture of Plantago lanceolata

Leaf and root explants from ۱۰ to ۲۰ day– old seedlings of Plantago lanceolata, were cultured on MS, MSH, NT and B۵ basal media with various concentrations of different plant growth regulators. The best callus induction was obtained with ۰.۴ mgl-۱ ۲,۴-D in MSH I with ۴۰۰ callus index. Callus growth was significantly stimulated (P=۰.۰۵)with ۰.۸ mgl-۱ ۲,۴-D and ۰.۱ mgl-۱ Kin in MSH II, with ۷۹۹ mg fresh weight and ۷۹ mg dry weight. A higher production of mucilage was obtained in MSH II. The mucilage content of different media varied from ۱۰.۴۰ to ۱۴.۷۳% dry weight. Perusal of the data reveals significant differences with regard to the total mucilage content. Callus has nearly ۳ times and ۱.۵ times more mucilage than seeds and leaf and root parts respectively.

Keywords:

Tissue culture , Mucilage , Plantago lanceolata