Molecular evaluation of sheep and goats isolates of Pasteurella multocida and their antibiotic resistance
Publish place: Veterinary Research Forum، Vol: 14، Issue: 9
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
View: 40
This Paper With 7 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_VRFAN-14-9_003
تاریخ نمایه سازی: 25 آبان 1402
Abstract:
Pasteurella multocida exists as a commensal in the upper respiratory tracts of livestock, and poultry, and causes a wide variety of diseases in humans and animals. This study aimed to investigate the incidence of P. multocida by bacteriological and molecular characterization in sheep and goats and screening the existence of capsule-specific genes and their antibiotic resistance pattern. Totally, ۱۶۵۰ nasopharyngeal swabs were collected from apparently healthy sheep and goats and ۴۶۰ lung tissues were collected from slaughtered animals in Fars province, Iran. All samples were cultured and suspected colonies were examined by biochemical tests, antimicrobial assay and polymerase chain reaction (PCR). Among ۱۶۵ P. multocida (۱۰۴ sheep and ۶۱ goats) isolates, the capA, capD, and capB genes were amplified in ۹۸, ۴۸, and ۱۲ isolates, respectively. The occurrence of four virulence-associated genes of P. multocida isolates were determined by PCR. Most isolates harbored the toxA (۷۹.۴۰%) and hgbB genes (۷۰.۹۰%) and ۵۹.۴۰% of isolates had the pfhA gene. Almost half of the isolates (۴۶.۱۰%) contained the tbpA gene. According to the current study, P. multocida capsular type A had the most frequency followed by type D. In addition, the high frequency of tbpA, pfhA, toxA, and hgbB genes revealed that these genes are possibly important in the pathogenesis of P. multocida. Oxytetracycline, enrofloxacin, florfenicol, and tilmicosin were the most effective drugs.
Keywords:
Authors
Mohammad Tabatabaei
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Fateme Abdolahi
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :