Human DT-diaphorase expression in Escherichia coli: optimization, purification and structural stability

Expression and purification of human DT-diaphorase, also referred to as NAD(P)H quinone oxidoreductase ۱ (NQO۱; EC. ۱.۶.۹۹.۲), which is a flavoprotein belongs to the family of oxidoreductases are optimized. The DT-diaphorase plays an important role in biosensor design for laboratory analysis and also developing biosensor for measurement of glucose level in blood. The aim of this study was to investigate various parameters regarding the expression of DT-diaphorase in Escherichia coli BL۲۱ (DE۳) and thermal stability of DT-diaphorase activity at different temperatures in the presence of sucrose. Expression conditions of DT-diaphorase in E. coli were optimized with an induction time (۲۲.۰۰ hr), induction temperature (۱۸.۰۰ ˚C) and also lactose (۵.۰۰ mM) and isopropyl ß-D-۱-thiogalactopyranoside (۱.۰۰ mM) concentrations as inducers. The Km, Vmax and kcat values for NADH as a substrate were ۲۵.۵۰ µM, ۳۵۷ µM per min and ۴۴۶.۴۰ μM mg-۱ per min, respectively. Results of our research revealed that different concentrations of sucrose at ۴۰.۰۰ ˚C did not have any significant effect on enzyme structure; while, relatively significant changes, especially in the presence of sucrose (۰.۷۵ M) at ۵۰.۰۰ ˚C were observed. The results presented show that sucrose causes DT-diaphorase inactivation rate reduction and relatively little increases in thermal stability and thus, sustains its conformation against thermal unfolding.

Keywords:

DT-diaphorase , thermal stability , Sucrose Osmolyte , Nitroblue tetrazolium