Genotypic evaluation of Pasteurella multocida isolated from cattle and sheep by pulsed-field gel electrophoresis

Pasteurella multocida a Gram-negative bacterium exists as a commensal in the upper respiratory tracts of livestock, and poultry. It is causative agent of a range of diseases in mammals and birds including fowl cholera in poultry, atrophic rhinitis in pigs and bovine hemorrhagic septicemia in cattle and buffalo. This study aimed to isolate P. multocida from sheep and cattle lungs sampled and assessed by bacteriological procedures and pulse field gel electrophoresis (PFGE) characterization. In this study ۵۲ isolated of P. multocida were obtained (۲۰۱۶-۲۰۱۷) from clinically healthy and diseased animals (sheep and cattle) evaluated by PFGE for determining the relationship between them. According to the results of this study ۱۲ sheep isolates had similarities above ۹۴.۰۰% and two cattle isolates showed similarities above ۹۴.۰۰%. When compared between sheep and cattle, most isolates showed a similarity of less than ۵۰.۰۰% indicating the great differences between isolates. It is noteworthy that in the present study, performed by PFGE to determine the type of P. multocida isolates, a very high distinction was made to determine the type of isolates and the relationship between isolates based on fragments in their genome using enzymes.

Keywords:

Pasturella mutocida , Pasteurellosis , PFGE Shiraz