Telomerase Activity in Chicken Embryo Fibroblast Cell Cultures Infected with Marek's Disease Virus
Publish place: Middle East Journal of Cancer، Vol: 1، Issue: 3
Publish Year: 1389
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_MISJ-1-3_005
تاریخ نمایه سازی: 25 آبان 1402
Abstract:
Background: Telomerase is a ribonucleoprotein, which adds telomeric repeats onto the ۳’ end of existing telomers at the end of chromosomes in eukaryotes. One hypothesis states that telomere length may function as a mitotic clock, therefore expression of telomerase activity in cancer cells may be a necessary and essential step for tumor development and progression.Methods: The detectability of telomerase activity in chicken embryo fibroblast (CEF) cells infected with different passages of Marek's disease virus (MDV) was tested with the TRAPEZE® telomerase detection kit at passages ۱۴ (P۱۴), P۸۰/۱ and P۱۲۰ for the Woodland strain, and passage ۹ (P۹) for the MPF۵۷ strain.Results: The results showed increased telomerase activity in MDV Woodlands strain at P۱۴ and MPF۵۷ strain at P۹.Conclusion: Our results suggest that MDV-transformed cells at low passage are a suitable system for the study of telomerases in tumor development and for testing telomerase-inhibiting drugs.
Authors
Mohammad Motamedifar
Department of Bacteriology and Virology, Shiraz HIV/AIDS Research Center (SHARC), Shiraz University of Medical Sciences, Shiraz, Iran
Gregory Tannock
Department of Applied Biology and Biotechnology, RMIT University, Melbourne, Australia